Deeping in our project

Hi Team:

Last session was incredible again. After an explanation to 2e team (parents, teachers, sisters, coordinators etc…); we have a more clear picture where we must reach on Spring next year. 

Almost a representative “sample” of 2e PIIISA 2016 project  team.

In resources I attached the presentation of last day (go to the presentation). And also you can see a link to the video of the PIIISA project 2014 (5 min) we project in order to see an example of a successful PIIISA research performed (http://piiisaandalucia.blogspot.com.es/p/que-es-piiisa.html ).

1^st experiment

Respect to the experiment of the first session related to determine how many bacteria are in a single colony of Sinorhizobium meliloti. We could obtain some conclusions, couldn’t we?. Think about them!!:

Some examples of the plates:

Cristina’s home (Placa 5)

Paula’s Home (placa 7)

We counted colonies and performed  this excel  table:

Under these data we can conclude that:

A single colony of S meliloti contains:   4x10⁵ – 2x10⁶ bact/colony. 

The number looks like depending  of their size.  These data are in agreement with the expected numbers (see previous blogs…). We also discuss about the reasons of this variability. Variability we have to take in account when we perform next mutagenesis experiments … .

Now!!  we know that, among other things, our project deals with counting bacteria!!

Also we tried to understand why some houses look better for bacterial incubation experiments than others (Cristina, and Paula’s home).

At the end of the session, we did bacterial streaking on agar plates in order to obtain single colonies of our experimental strains. Remember 3G and 3I. Remind this tutorial to understand how single colonies must appear from our plates. https://www.youtube.com/watch?v=X_0e9WuFTrQ 

Next session we’ll perform our first mutation study.  

We keep in contact!!