After 8 days (6 days of bacterial cultivation at Paula’s home). We have our first numbering results. We made plating and we have colonies for counting.
Fig 1: Lab sacharosa plates
Fig2: Lab TY plates
Fig3: Paula’s Sac Plates
Fig 4: Paula’s TY plates
Watching our results it is difficult to interpret a mutation frequency, isn’t it?
Of course, dilution 0 of Sac plates cannot be counted (we have to discuss what occurs in such plates) but, what about the others?
If you analyze the plates you find out there is a good correlation between lab data and Paula’s home data. We can fill in a table those numbers and estimate the mutation frequency:
Number of colonies Sac Resistant/Number of colonies of total culture.
Again we must remind the dilutions we performed (see previous blog entries)
Students!!! We have as home work this Christmas to estimate the basal mutation frequency of 3G isolate based on Lab plates and Paula’s plates Also see the images of the wassaps!!!.
Is it 10-4, 10-5, 10-6, 10-7, zero??
Please use the blog to explain our results!!!. Compare then with the previous blog. We can assume that our results are reproducible (also we can estimate error margins).
Again have a Nice Christmas Time!!!
Francisco Martínez-Abarca
Departamento de Microbiología y Sistemas Simbióticas.
Estación Experimental del Zaidín. CSIC.
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