Last Results, First Conclusions

Hi Team!!!

Again, last session dealt with our favourite activity: “Counting colonies”, isn’t it??

Of course, we did more things!!!. We get it our last (final) result (sumarizes on  table 1).

Table 1: bacterial colony counting of the last two experiments. Dilutions (-4)-(-6) correspond total colonies on TY Km plates; Dilutions (1) to (-1) correspond total colonies on TY Km, Sac plates.

When we use those data to get the graphic of uv effect on bacterial growth; we decide to not consider the data on 3G bacteria plates. It is probably some error  in the dilution protocol performed with this particular isolate. However, a nice and reproducible uv effect on bacterial culture can be observed in the experiments I and II (3I bacterium isolate). From those data we can obtain our results and conclusions of our project (see Figure 1).

Figure 1: Effect of uv treatment on (A) bacterial growth and (B) mutation frequency of S. meliloti bacteria.

All of us must be able to obtain the numbers of this figures based on table 1; understand their meaning and of course understand which are our conclusions:

1. We demonstrate S meliloti growth is sensitive to uv treatments
2. Uv treatments does not increase the mutation frequency in S. meliloti.
3. We cannot discard a putative effect on the type of mutation generated.

Now we have to:

1. Put the figure legend on all figures of the work: (see in resouces Figures) see examples on previous PIIISA manuscripts.
2. Think in the my own ideas section.

Based on our work: manuscript (proceedings), Poster and Oral presentation must be designed.

Come on!!! we almost touching the end of this great adventure!!!!

Francisco Martínez-Abarca

Departamento de Microbiología y Sistemas Simbióticos.

Estación Experimental del Zaidín. CSIC.

Granada.